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1.
West China Journal of Stomatology ; (6): 556-559, 2010.
Article in Chinese | WPRIM | ID: wpr-350285

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the effect of Astragalus membranaceus (APS) on the proliferation, osteogenic capacity and structure of periodontal ligament cells (PDLCs) in vitro.</p><p><b>METHODS</b>PDLCs were cultured in vitro with APS of 0.08, 0.1, 0.2, 0.4 mg x mL(-1). Methyl thiazolyl tetrazolium (MTr), alkaline phosphatase (ALP) and cell structure were detected to determine the proliferation and differentiation of PDLCs proliferation and differentiation.</p><p><b>RESULTS</b>When the APS was 0.2 mg x mL(-1), the absorbance of MTT and ALP exhibit significantly increased as compared to the control (P < 0.05). The cells cultured in vitro with APS of 0.2 mg x mL(-1) had the normal structure.</p><p><b>CONCLUSION</b>APS with proper concentration in short-term culture may promote the proliferation and differentiation of PDLCs.</p>


Subject(s)
Alkaline Phosphatase , Astragalus propinquus , Cell Differentiation , Cell Proliferation , In Vitro Techniques , Periodontal Ligament
2.
West China Journal of Stomatology ; (6): 149-157, 2010.
Article in Chinese | WPRIM | ID: wpr-246635

ABSTRACT

<p><b>OBJECTIVE</b>To evaluate the effect of different concentrations of ethylenediamine tetraacetic acid (EDTA) paste on the removing root canal smear layers and the degrees of erosion on the surface of the root canal walls at the different portions of canal.</p><p><b>METHODS</b>Sixty human teeth with single root were instrumented using step-back technique, then were divided into six groups and treated with different concentrations EDTA paste and NaCLO solution. Group A: 0.9% saline; group B: 5.25% NaC10+5% EDTA; group C: 5.25% NaC10+10% EDTA; group D: 5.25% NaC10+15% EDTA; group E: 5.25% NaC10+17% EDTA; group F: 5.25% NaClO+20% EDTA. Then the teeth were split, the root canals with different treatments were examined with scanning electron microscope at the coronal, middle and apical thirds for smear layers removal and the degrees of erosion.</p><p><b>RESULTS</b>The effect of EDTA paste on removing smear layers at the coronal, middle thirds of the root canal increased with the concentrations. 15% EDTA paste could remove the smear layers at the coronal and middle thirds of the root canal, but the effect on apical third was invalid, and no erosion could be found in the root canal wall. 17% and 20% EDTA paste produced the erosion to the root canal wall.</p><p><b>CONCLUSION</b>The concentrations of the EDTA paste can influence the effect of the removing smear layers, and the concentration beyond 17% would produce the erosion to root canal wall.</p>


Subject(s)
Humans , Dental Pulp Cavity , Edetic Acid , Microscopy, Electron, Scanning , Ointments , Root Canal Irrigants , Root Canal Preparation , Root Canal Therapy , Smear Layer
3.
Chinese Journal of Radiology ; (12)2001.
Article in Chinese | WPRIM | ID: wpr-680029

ABSTRACT

Objective To evaluate the difference of internal diameter of bronchial artery in big lung cancer,small lung cancer,and normal lung with multiple slice CT.Methods MSCT angiographies of 44 patients with lung cancer confirmed by pathology were retrospectively analyzed,and 29 patients were with big lung cancer(≥3 cm)and 15 patients with small lung cancer(

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